Publications

RESEARCH PAPERS

Wood, D.C., Jurgensen, S.R., Geesin, J.C. and Harrison, J.H. "Subunit interactions in mitochondrial malate dehydrogenase: kinetics and mechanism of reassociation." J. Biol. Chem.  (1981) 256:2377-2382.

Schechter, N.M., Frški, J.E., Geesin, J.C. and Lazarus, G.S. "Human skin chymotryptic proteinase: isolation and relation to Cathepsin G and Rat Mast Cell Proteinase I." J. Biol. Chem. (1983) 258:2973-2978.

Geesin, J.C., Murad, S. and Pinnell, S.R. "Ascorbic acid stimulates collagen production without altering intracellular degradation in cultured human skin fibroblasts." Biochim. Biophys. Acta (1986) 886:272-274.

Geesin, J.C., Darr, D., Kaufman, R., Murad, S. and Pinnell, S.R. "Ascorbic acid specifically increases type I and type III procollagen messenger RNA levels in human skin fibroblasts." J. Invest. Dermatol. (1988) 90:420-424.

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Retinoids affect collagen synthesis through inhibition of ascorbate induced lipid peroxidation in cultured human dermal fibroblasts." Arch. Biochem. Biophys. (1990) 278:350-355.

Jacobson, T.M., Y‹ksel, K.U., Geesin, J.C., Gordon, J.S., Lane, A.T. and Gracy, R.W. "Effects of aging and xerosis on the amino acid composition of human skin." J. Invest. Dermatol. (1990) 95:296-300.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Modulation of collagen synthesis by growth factors: the role of ascorbate-stimulated lipid peroxidation." Arch. Biochem. Biophys. (1991) 289:6-11.

Geesin, J.C., Hendricks, L.J., Falkenstein, P.A., Gordon, J.S. and Berg, R.A. "Regulation of collagen synthesis by ascorbic acid: characterization of the role of  ascorbate-stimulated lipid peroxidation." Arch. Biochem. Biophys. (1991) 290:127-132.

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Regulation of collagen synthesis in human dermal fibroblasts by the sodium and magnesium salts of ascorbyl-2-phosphate." Skin Pharmacol. (1993) 6:65-71.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Regulation of collagen synthesis in human dermal fibroblasts in contracted collagen gels by ascorbic acid, growth factors and inhibitors of lipid peroxidation." Exp. Cell Res. (1993) 206:283-290.

Brown, L.J., Rothnagel, J., Geesin, J.C., Roop, D.R. and Gordon, J.S. "Retinoid suppression of loricrin expression in reconstituted human skin cultured at the liquid-air interface." J. Invest. Dermatol. (1994) 102:886-890.

Geesin, J.C., Brown, L.J., Liu, Z. and Berg, R.A. "Development of a skin model based on insoluble fibrillar collagen." J. Appl. Biomater. (1996) 33:1-8.

Cullen, B., Silcock, D., Brown, L.J., Gosiewska, A. and Geesin, J.C. "The differential regulation and secretion of proteinases from fetal and neonatal fibroblasts by growth factors." Internat. J. Biochem. Cell Biol. (1997) 29:241-250.

Gosiewska, A., Yi, C.-Y., Blanc-Brude, O. and Geesin, J.C. "Characterization of a macrophage-based system for studying the activation of latent TGF-Ŗ." Methods in Cell Science (1999) 21:47-56.

Yi, C.-Y., Gosiewska, A., Burtis, D. and Geesin, J. "The incorporation of fluorescent enzyme substrates in agarose gel for in situ zymography." Analytical Biochemistry (2001) 291:27-33.

Gosiewska, A., Rezania, A., Dhanaraj. S., Vyakarnam, M., Zhou, J., Burtis, D., Brown, L., Kong, W., Zimmerman, M. and Geesin, J.C. "Development of a 3-dimensional (3-D) transmigration assay for testing cell-polymer interactions for tissue engineering applications." Tissue Engineering (2001) 7:267-277.

Gosiewska, A., Yi, C.-Y., Brown, L., Cullen, B. and Geesin, J.C. "Differential expression and regulation of extracellular matrix-associated genes in fetal and neonatal fibroblasts." Wound Repair & Regeneration (2001) 9:216-225.

Dijkman, J., Xu, J., Masure, S., Dhanaraj, S., Gosiewska, A., Geesin, J., Sprengel, J., Harris, S., Verhasselt, P. Gordon, R. and Yon, J. "Characterization of platelet-derived growth factor-C (PDGF-C): expression in normal and tumor cells, biological activity and chromosomal localization." International Journal of Biochemistry and Cell Biology  (2002) 34:414-426.

CHAPTERS

Geesin, J.C. and Berg, R.A. "Biochemistry of skin, cartilage and bone." in Clinical Use of Biomaterials in Facial Plastic Surgery, F.H. Silver and A. Glasgold, eds. CRC Press: Boca Raton, Florida (1991) pp. 7-27. 

Rucker, R., Geesin, J.C. and Berg, R.A. "Vitamin C." in Handbook of Vitamins. R.B.  Rucker, J.W. Suttie, D.B. McCormack and L.J. Machlin, eds. Marcel Dekker, Inc.: New York (2001) pp. 555-568.

Holy, Chantal E., Volenec, Jerry, Geesin, Jeffrey and Bruder, Scott P. "Bone Regeneration" in Principals of Tissue Engineering Chapter 56, 2007, Elsevier.

ABSTRACTS

Schechter, N.M., Frški, J.E., Briggaman, R.A., Geesin, J.C. and Lazarus, G.S. "Comparison of the human skin chymotrypsin-like proteinase with Cathepsin G and its location within skin." Fed. Proc. (1982) 41:507.

Frški, J.E., Schechter, N.M., Briggaman, R.A., Geesin, J.C. and Lazarus, G.S. "Comparison of the human skin chymotrypsin-like proteinase with Cathepsin G and its location within skin." Clin. Res. (1982) 30:584.

Geesin, J.C., Murad, S. and Pinnell, S.R. "Stimulation of collagen production in human skin fibroblasts by ascorbic acid is unrelated to intracellular degradation." Clin. Res. (1985) 33:639.

Darr, D.J., Geesin, J.C. and Pinnell, S.R. "Ascorbic acid specifically stimulates type I and type III collagen mRNA." J. Invest. Dermatol. (1986) 86:470.
  
Jacobson, T.M., Gracy, R.W., Lane, A.T., Gordon, J.S. and Geesin, J.C. "Amino acid changes in dry skin syndrome." J. Cell Biol. (1988) 107:295a.

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Retinoids inhibit collagen synthesis through inhibition of ascorbate stimulated lipid peroxidation." J. Invest. Dermatol. (1989) 92:433.  

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Ascorbic acid stimulates collagen synthesis through its ability to cause lipid peroxidation in human dermal fibroblasts." Clin. Res. (1989) 37:350A.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Ascorbate modulates the activity of FGF but not TGF-Ŗ on collagen synthesus in human dermal fibroblasts." FASEB J. (1990) 4:A2286.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Lipid peroxidation stimulates collagen synthesis in human dermal fibroblasts." Free Radical Biol. Med. (1990) 9:4.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Ascorbate modulates the activity of FGF but not TGF-Ŗ on collagen synthesis in human dermal fibroblasts." Matrix: Collagen Rel. Res. (1990) 10:255-256.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Regulation of collagen synthesis in dermal fibroblasts by ascorbate-induced lipid peroxidation." FASEB J. (1991) 5:A445.

Hendricks, L.J., Geesin, J.C., Roop, D.R. and Gordon, J.S. "Retinoid suppression of  loricrin expression in reconstituted human skin cultured at the liquid-air interface." J. Invest. Dermatol. (1991) 96:548.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Regulation of collagen synthesis in human dermal fibroblasts in contracted collagen gels by ascorbic acid." J. Invest. Dermatol. (1991) 96:607.

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Regulation of collagen synthesis in human dermal fibroblasts by the sodium and magnesium salts of ascorbyl-2-phosphate." J. Invest. Dermatol. (1991) 96:607.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Regulation of collagen synthesis in human dermal fibroblasts in contracted collagen gels by ascorbic acid, growth factors and inhibitors of lipid peroxidation." FASEB J. (1992) 6:A353.

Geesin, J.C., Brown, L.J., Gordon, J.S. and Berg, R.A. "Development of a skin model based on insoluble fibrillar collagen." J. Invest. Dermatol. (1992) 98:623.

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Magnesium ascorbyl-2-phosphate is a more stable alternative to ascorbic acid in the stimulation of collagen synthesis in human dermal fibroblasts." J. Invest. Dermatol. (1992) 98:623.

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Lipid peroxidation inhibitors regulate the synthesis of messenger RNA for types I and III collagen in dermal fibroblasts." Matrix: Collagen Rel. Res. (1993) 13:65.

Berg, R.A., Geesin, J.C., Brown, L.J. and Gordon, J.S. "Ascorbate induced collagen synthesis in human dermal fibroblasts through a pro-oxidant mechanism." J. Cell. Biochem. (1993) 17E:125.

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Ascorbic acid stimulated collagen gene expression is prevented by transient transfection of human dermal fibroblasts." J. Invest. Dermatol. (1993) 100:549.

Brown, L.J., Rothnagel, J.A., Geesin, J.C., Roop, D.R. and Gordon, J.S. "The effect of  retinoic acid on keratin 1 and loricrin expression in reconstituted human skin cultured at the liquid-air interface." J. Invest. Dermatol. (1993) 100:580.

Geesin, J.C., Nebus, J.A. and Cole, C.A. "Characterization of the action spectrum and cell-type dependence for UVA-induced  lipid peroxidation in cultured cells." J. Invest. Dermatol. (1994) 102:601.

Geesin, J.C., Wildnauer, T., Nebus, J.A. and Cole, C.A. "Prevention of ultraviolet-induced oxidative effects on cultured fibroblasts by antioxidants." J. Invest. Dermatol. (1997) 108:665.

Geesin, J.C., Nebus, J.A. and Cole, C.A. "Effect of retinoids on ultraviolet-mediated oxidative effects on cultured fibroblasts." J. Invest. Dermatol. (1997) 108:579.

Gosiewska, A., Yi, C.-F., Blanc-Brude, O., Huang, J. and Geesin, J.C. "In vitro characterization of the antifibrotic potential of mannose-6-phosphate." Wound Repair and Regeneration (1997) 5:A112.

Gosiewska, A., Brown, L.J., Yi, C.-F., Cullen, B. and Geesin. J.C. "The intrinsic differences between fetal and neonatal human dermal fibroblasts." Wound Repair and Regeneration (1997) 5:A112.

Cullen, B., Silcock, D., Brown, L.J., Gosiewska, A. and Geesin, J.C. "Differential regulation of fetal and neonatal fibroblast-mediated collagen gel contraction in the presence or absence of exogenous growth factors." Wound Repair and Regeneration (1997) 5:A110.

Gosiewska, A., Yi, C.-Y., Brown, L., and Geesin, J.C. "The effect of enzyme debriders on the biological activity of recombinant human platelet-derived growth factor-BB (rhPDGF-BB), the active agent of Regranex Gel." Wound Repair and Regeneration (1998) 6:A254.

Gosiewska, A., Rezania, A., Dhanaraj, S., Vyakarnam, M., Zhou, J., Burtis, D., Brown, L., Kong, W., Zimmerman, M. and Geesin, J.C. "Development of a 3-dimensional transmigration assay for testing cell-polymer interactions for tissue engineering applications." Wound Repair and Regeneration (2001) 9:143.

Yi, C.-Y., Gosiewska, A., Burtis, D. and Geesin, J. "The incorporation of fluorescent enzyme substrates in agarose gel for in situ zymography." Wound Repair and Regeneration (2001) 9:164.

UNPUBLISHED PRESENTATIONS

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Retinoids inhibit collagen synthesis through inhibition of ascorbate-stimulated lipid peroxidation." Presented at the Johnson & Johnson Skin Care Technology Workshop, June 1989.

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Ascorbic acid stimulates collagen synthesis through its ability to cause lipid peroxidation in human dermal fibroblasts." presented at the Collagen Gordon Conference, July 1989.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Modulation of collagen synthesis in human dermal fibroblasts: the role of ascorbate-stimulated lipid peroxidation." presented at the East Coast Connective Tissue Meeting, April 1990.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Modulation of collagen synthesis by growth factors: the role of ascorbate-stimulated lipid peroxidation." presented at the Peptide Growth Factor Gordon Conference, July 1990.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Regulation of collagen synthesis in human dermal fibroblasts in contracted collagen gels by ascorbic acid." presented at the East Coast Connective Tissue Meeting, March 22-23, 1991.

Geesin, J.C., Hendricks, L.J., Gordon, J.S. and Berg, R.A. "Regulation of collagen synthesis in dermal fibroblasts by ascorbate-induced lipid peroxidation." presented at the East Coast Connective Tissue Meeting, March 22-23, 1991.

Hendricks, L.J., Geesin, J.C., Gordon, J.S. and Roop, D.R. "Retinoid suppression of loricrin expession in reconstituted human skin cultured at the liquid-air interface." presented at the Gordon Conference on Epithelial Differentiation, August, 1991.
  
Geesin, J.C., Brown, L.J., Gordon, J.S. and Berg, R.A. "Development of a skin model based on insoluble fibrillar collagen." presented at the First New Jersey Symposium on Biomaterials and Medical Devices, March 18, 1992.  

Berg, R.A., Geesin, J.C., Brown, L.J. and Gordon, J.S. "Regulation of collagen synthesis in dermal equivalents by ascorbic acid, fibroblast growth factor and transforming growth factor-beta." presented at the Keystone Symposia on Tissue Engineering, April, 1992.

Geesin, J.C., Brown, L.J., Gordon, J.S. and Berg, R.A. "Characterization of the role of ascorbate-stimulated lipid peroxidation in ascorbate-stimulated collagen synthesis." presented at the FASEB Summer Conference on Vitamin C and Vitamin E in Free Radical Reactions, July 19-24, 1992.

Geesin, J.C., Gordon, J.S. and Berg, R.A. "Ascorbic acid stimulated collagen gene expression is prevented by transient transfection of human dermal fibroblasts." presented at the East Coast Connective Tissue Society Annual Meeting, March 19 & 20, 1993.
  
Geesin, J.C., Gordon, J.S. and Berg, R.A. "Ascorbic acid stimulated collagen gene expression is prevented by transient transfection of human dermal fibroblasts." presented at the Wound Repair Gordon Conference, June 14-18, 1993.

Geesin, J.C., Brown, L.J., Gordon, J.S. and Berg, R.A. "Characterization of the role of ascorbate-stimulated lipid peroxidation in ascorbate-stimulated collagen synthesis." presented at the Wound Repair Gordon Conference, June 14-18, 1993.

Geesin, J.C., Cullen, B.M., Brown, L.J., Gosiewska, A. and Yi, C.-F. "In vitro phenotypic differences between fetal and neonatal human dermal fibroblasts." presented at the Australian Wound Management Association's National Conference, February 23, 1996.

Geesin, J.C. and Berg, R.A. "In vitro regulation of collagen synthesis by ascorbic acid: possible mechanisms and importance to wound repair." presented at the Australian Wound Management Association's National Conference, February 23, 1996.

Geesin, J.C., Cullen, B.M., Brown, L.J., Gosiewska, A. and Yi, C.-F. "In vitro phenotypic differences between fetal and neonatal human dermal fibroblasts." presented at the Therapeutic Advances in Fibrosis Conference, April 18-19, 1996.

Geesin, J.C. "In vitro characterization of the anti-fibrotic potential of mannose-6-phosphate." Presented at the University College London, March 18th, 1997.

Geesin, J.C. "The use of in vitro engineered skin models to assess potential wound healing therapies." presented at the Symposium Commemorating the Retirement of Professor Nobuyuki Shioya, March 29, 1997 in Yokohama, Japan.

Geesin, J. "In vitro characterization of the anti-fibrotic potential of mannose-6-phosphate."  presented at the IBC's Second International Conference on Fibrosis, April 28-29, 1997 in Washington, DC.

Gosiewska, A., Yi, C.-Y., Brown, L., and Geesin, J.C. "The effect of enzyme debriders on the biological activity of recombinant human platelet-derived growth factor-BB (rhPDGF-BB), the active agent of Regranex Gel." Presented at the 8th Annual Meeting of the European Tissue Repair Society, August 27-30, 1998 in Copenhagen, Denmark.

Geesin, J.C. "Bringing a wound care product to market: preclinical/animal models." Presented at the Wound Healing Society Educational Symposium on Translational Research in Wound Care, June 22, 1999 in Minneapolis, MN.

Geesin, J.C. "Development of a novel rat model of wound ischemia." Presented at the Wound Healing Society Educational Symposium, Workshop on Preclinical Models, June 4, 2000 in Toronto, Canada.

Chang, J., Yi, C.-F., Smith, K., Dhanaraj, S., Burtis, D., Patel, K., Brown, L., Plouhar, P., Gosiewska, A. and Geesin, J. "TGF-Ŗ coated collagen/GAG matrices for cartilage tissue engineering." Presented at the Gordon Conference on "Signal Transduction by Engineered Extracellular Matrices" June 25-29, 2000 in New Hampshire.

Geesin, J.C. "Wound Biomaterials: Applications in Tissue Engineering." Presented at the Fourteenth Annual Symposium on Advanced Wound Care, Las Vegas, Nevada, on May 3rd, 2001.

Dhanaraj, S; Mukhopadhyay, K; Fung, R; Wang, Z; Gosiewska, A; Geesin, J; Plouhar, P; Bruder, S. "Stimulation of cell proliferation and cell migration by platelet rich plasma (PRP)." Presented at the Orthopaedic Research Society Conference in March 2004. 

Dhanaraj, S., Wang, Z., Geesin, J., Karr, M., and Bowman, S., "Chondrogenic redifferentiation of in vitro expanded human chondrocytes." Presented at the Regenerate: Tissue Engineering the Human Body Conference in Seattle, Washington, June 9-12, 2004 

Dhanaraj, S; Mukhopadhyay, K; Fung, R; Wang, Z; Gosiewska, A; Geesin, J; Plouhar, P; Bruder, S.  "Stimulation of cell proliferation and cell migration by platelet rich plasma (PRP)." Platform presentation at the "Regenerate- Tissue engineering the human body" in June 2004.

Cole, B., Lu, Y., Dhanaraj, S., Wang, Z. Bradley, D. Boock, R. and Binette, F. "A Single-Stage Cell Based Approach for the Treatment of Articular Cartilage Defects." Abstract submitted to AAOS for presentation in 2005.

Dhanaraj, S., Binette, F., Wang, Z., Lu, E., Hammer, J., Bradley, D., and Geesin, J.
"Chondrocyte Migration From Cartilage Fragments into Bioresorbable Scaffolds and Formation of de Novo Cartilage Following Implantation into SCID Mice." Submitted to the 2005 Orthopaedic Research Society Meeting in Washington, DC, February 2005.

Dhanaraj, S., Binette, F., Wang, Z., Shetty, D., Bradley, D., Lu, E., and Geesin, J. "Chondrocyte Migration From Cartilage Fragments into Bioresorbable Scaffolds is Dependent on the Size of Cartilage Fragments." Submitted to the 2005 Orthopaedic Research Society Meeting in Washington, DC, February 2005.

Dhanaraj, S., Binette, F., Wang, Z., Shetty, D., Lin, X., Lu, E., and Geesin, J.
Cartilage Fragments, But Not Bone Cartilage Paste Loaded Scaffolds, Form Uniform Cartilage Like Tissue Following Implantation into SCID Mice." Submitted to the 2005 Orthopaedic Research Society Meeting in Washington, DC, February 2005.

Muschler, G.F., Senechal, G., Fang, C., Holy, C.E., Geesin, J., Volenec, F.J. and Bruder, S.P. "Selective Cell Retention of Connective Tissue Progenitor Cells on a  Hydroxyapatite-Coated Collagen Matrix." ." Submitted to the 2005 Orthopaedic Research Society Meeting in Washington, DC, February 2005.

Geesin, J.C. "The Role of Big Pharma in Regenerative Medicine"; A Johnson & Johnson Perspective" Presented at 5th Annual Conference: Commercial Translation of Regenerative Medicine, London, UK on November 15 & 16, 2007.



Geesin Consulting
Geesin Consulting
[Home] [Resume] [Publications] [Patents]